Article
Genotyping and Quantifying Lyme Pathogen Strains by Deep Sequencing of the Outer Surface Protein C (ospC) Locus.
Journal of clinical microbiology - 1 Nov 2018
Di Lia, Wan Zhenmao, Akther Saymon, Ying Chunxiao, Larracuente Amanda, Li Li, Di Chong, Nunez Roy, Cucura D Moses, Goddard Noel L, Krampis Konstantino, Qiu Wei-Gang
Abstract excerpt
A mixed infection of a single tick or host by Lyme disease spirochetes is common and a unique challenge for the diagnosis, treatment, and surveillance of Lyme disease. Here, we describe a novel protocol for differentiating Lyme strains on the basis of deep sequencing of the hypervariable outer surface protein C locus (ospC). Improving upon the traditional DNA-DNA hybridization method, the next-generation...
Topics
- Animals
- Antigens, Bacterial
- Bacterial Load
- Bacterial Outer Membrane Proteins
- Borrelia
- Borrelia burgdorferi
- Coinfection
- DNA, Bacterial
- Female
- Genetic Variation