Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Rapid visual isothermal nucleic acid-based detection assay ofBrucellaspecies by polymerase spiral reaction

20 Apr 2018

Abstract excerpt

AIM: The aim of this study was to develop polymerase spiral reaction (PSR) for rapid, sensitive and specific detection of Brucella sp. METHODS AND RESULTS: Polymerase spiral reaction assay was developed using specifically designed primers targeting the conserved multicopy IS711 gene of Brucella sp. The assay could be performed within 60 min at an isothermal temperature of 64°C. The lower limit of detection of PSR...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Biosensors and Analytical Detection
Share to Brucella: diagnosis, epidemiology, treatment
Share to Burkholderia infections and melioidosis

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 30152897
DOI: 10.1111/jam.13882

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Rapid visual isothermal nucleic acid-based detection assay ofBrucellaspecies by polymerase spiral reactionPMID 30152897Read ↗

Diagnosis of human brucellosis using PCRSemantic proximity Read ↗

Evaluation of genus-specific and species-specific real-time PCR assays for the identification of Brucella spp.Semantic proximity Read ↗

Rapid detection of Brucella spp. by the loop-mediated isothermal amplification methodSemantic proximity Read ↗

Polymerase chain reaction–based assays for the diagnosis of human brucellosisSemantic proximity Read ↗

A novel, highly sensitive, one-tube nested quantitative real-time PCR for Brucella in human blood samplesSemantic proximity Read ↗

Differential diagnosis of Brucella abortus by real-time PCR based on a single-nucleotide polymorphisms.Semantic proximity Read ↗

Comparative study of IS711 and bcsp31-based polymerase chain reaction (PCR) for the diagnosis of human brucellosis in whole blood and serum samplesSemantic proximity Read ↗

Improving sensitivity of single tube nested PCR to detect fastidious microorganismsSemantic proximity Read ↗

Multiplex PCR Assay for the Identification and Differentiation of all Brucella Species and the Vaccine Strains Brucella abortus S19 and RB51 and Brucella melitensis Rev1Semantic proximity Read ↗

Evaluation of three polymerase chain reaction techniques for detection ofBrucellaDNA in peripheral human bloodSemantic proximity Read ↗

Highly Sensitive Bacteriophage-Based Detection of Brucella abortus in Mixed Culture and Spiked BloodSemantic proximity Read ↗

Serum Is the Preferred Clinical Specimen for Diagnosis of Human Brucellosis by PCRSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.