Evaluation of genus-specific and species-specific real-time PCR assays for the identification of Brucella spp.

Abstract excerpt

BACKGROUND: The identification of Brucella isolates using conventional microbiological techniques is time-consuming and hazardous. We therefore assessed the performance of real-time PCR assays for the identification of members of the genus Brucella to the genus and species level. METHODS: We evaluated an in-house developed assay and various previously published real-time PCR assays targeting bcsp31, per, IS711,...

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