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Rapid detection of Brucella spp. by the loop-mediated isothermal amplification method

1 Feb 2008

Abstract excerpt

AIMS: To develop a rapid and sensitive method for detecting Brucella spp. METHODS AND RESULTS: Two sets of six Brucella-specific primers for loop-mediated isothermal amplification (LAMP) were designed from the sequence of the Brucella abortus BCSP31 gene. The specificity and sensitivity were examined for six Brucella species (22 strains) and 18 non-Brucella species (28 strains). The LAMP assay was specific to...

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PMID: 18248366
DOI: 10.1111/j.1365-2672.2008.03732.x

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