Article
[Effect of superhelical DNA on the transcription of cloned genes of the T4 phage].
Molekuliarnaia biologiia - 1 Jan 2000
Ogryz'ko V V, Mirkin S M, Shmerling Zh G, Siniakova R N, Zograf Iu N
Abstract excerpt
The effect of DNA superhelicity on the transcription of T4 DNA fragments containing early genes uvs W, Y and late genes 25-29 was studied. RNA polymerase transcribes both early and late phage genes within the supercoiled recombinant plasmid. Late genes relative transcription increases essentially...
Topics
- Cloning, Molecular
- DNA Topoisomerases, Type II
- DNA, Recombinant
- DNA, Superhelical
- DNA-Directed RNA Polymerases
- Escherichia coli
- Genes, Viral
- Mutation
- Plasmids
- T-Phages
- Transcription, Genetic