Article
[Effect of superhelical DNA on the transcription of cloned genes of the T4 phage].
Molekuliarnaia biologiia - 1 Jan 2000
Ogryz'ko V V, Mirkin S M, Shmerling Zh G, Siniakova R N, Zograf Iu N
Abstract excerpt
The effect of DNA superhelicity on the transcription of T4 DNA fragments containing early genes uvs W, Y and late genes 25-29 was studied. RNA polymerase transcribes both early and late phage genes within the supercoiled recombinant plasmid. Late genes relative transcription increases essentially when T4-modified RNA polymerase is used. DNA relaxation causes a sharp decrease of modified RNA polymerase activity,...
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