Article
Tracing mother-infant transmission of bacteriophages by means of a novel analytical tool for shotgun metagenomic datasets: METAnnotatorX.
Microbiome - 20 Aug 2018
Milani Christian, Casey Eoghan, Lugli Gabriele Andrea, Moore Rebecca, Kaczorowska Joanna, Feehily Conor, Mangifesta Marta, Mancabelli Leonardo, Duranti Sabrina, Turroni Francesca, Bottacini Francesca, Mahony Jennifer, Cotter Paul D, McAuliffe Fionnuala M, van Sinderen Douwe, Ventura Marco
Abstract excerpt
BACKGROUND: Despite the relevance of viral populations, our knowledge of (bacterio) phage populations, i.e., the phageome, suffers from the absence of a "gold standard" protocol for viral DNA extraction with associated in silico sequence processing analyses. To overcome this apparent hiatus, we present here a comprehensive performance evaluation of various protocols and propose an optimized pipeline that covers...
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