Article
A Sequel to Sanger: amplicon sequencing that scales.
BMC genomics - 27 Mar 2018
Hebert Paul D N, Braukmann Thomas W A, Prosser Sean W J, Ratnasingham Sujeevan, deWaard Jeremy R, Ivanova Natalia V, Janzen Daniel H, Hallwachs Winnie, Naik Suresh, Sones Jayme E, Zakharov Evgeny V
Abstract excerpt
BACKGROUND: Although high-throughput sequencers (HTS) have largely displaced their Sanger counterparts, the short read lengths and high error rates of most platforms constrain their utility for amplicon sequencing. The present study tests the capacity of single molecule, real-time (SMRT) sequencing implemented on the SEQUEL platform to overcome these limitations, employing 658 bp amplicons of the mitochondrial...
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