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Article

Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I.

The Journal of veterinary medical science - 27 Apr 2018

Okino Cintia Hiromi, Montassier Maria de Fátima Silva, Oliveira Andressa Peres de, Montassier Helio José

Abstract excerpt

A method based on Melting Temperature analysis of Hypervariable regions (HVR) of S1 gene within a RT-qPCR was developed to detect different genotypes of avian infectious bronchitis virus (IBV) and identify the Mass genotype. The method was able to rapidly identify the Mass genotype among IBV field isolates, vaccine attenuated strains and reference M41 strain in allantoic liquid and also directly in tissues. The...

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Identifiers

PMID: 29491226
DOI: 10.1292/jvms.17-0566
PMCID: PMC5938207

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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