Article
Structural stability of purified human CFTR is systematically improved by mutations in nucleotide binding domain 1.
Biochimica et biophysica acta. Biomembranes - 1 May 2018
Yang Zhengrong, Hildebrandt Ellen, Jiang Fan, Aleksandrov Andrei A, Khazanov Netaly, Zhou Qingxian, An Jianli, Mezzell Andrew T, Xavier Bala M, Ding Haitao, Riordan John R, Senderowitz Hanoch, Kappes John C, Brouillette Christie G, Urbatsch Ina L
Abstract excerpt
The Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) is an ABC transporter containing two transmembrane domains forming a chloride ion channel, and two nucleotide binding domains (NBD1 and NBD2). CFTR has presented a formidable challenge to obtain monodisperse, biophysically stable protein. Here we report a comprehensive study comparing effects of single and multiple NBD1 mutations on stability of both...
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