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Article

Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9.

Stem cell research - 1 Jan 2018

Yuan Fang, Guo Dongsheng, Liu Yanli, Xu Yingying, Gao Ge, Wu Yuhang, Yang Fan, Ke Xinrong, Lai Keyu, Wei Hongcheng, Li Yin-Xiong

Abstract excerpt

The ASS1 gene encodes argininosuccinate synthetase-1, a cytosolic enzyme with a critical role in the urea cycle. Mutations are found in all ASS1 exons and cause the autosomal recessive disorder citrullinemia. Using CRISPR/Cas9-editing, we established the WAe001-A-13 cell line, which was heterozygous for an ASS1 mutation, from the human embryonic stem cell line H1. The WAe001-A-13 cell line maintained the...

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Identifiers

PMID: 29247816
DOI: 10.1016/j.scr.2017.11.007

Genes

ASS1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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