Article
An autofluorescence-based method for the isolation of highly purified ventricular cardiomyocytes.
Cardiovascular research - 1 Mar 2018
Larcher Veronica, Kunderfranco Paolo, Vacchiano Marco, Carullo Pierluigi, Erreni Marco, Salamon Irene, Colombo Federico Simone, Lugli Enrico, Mazzola Marta, Anselmo Achille, Condorelli Gianluigi
Abstract excerpt
AIMS: The aim of our study was to set up a simple and reliable isolation method of living ventricular cardiomyocytes (vCMs) for molecular and biological studies. METHODS AND RESULTS: A standard technique for the retrograde perfusion of an enzymatic solution was used to isolate cardiac cells from adult mouse heart. Fluorescence-activated cell sorting (FACS) on adult murine cardiac ventricle cells was performed,...
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