Article
OMIP-099: 31-color spectral flow cytometry panel to investigate the steady-state phenotype of human T cells.
Cytometry. Part A : the journal of the International Society for Analytical Cytology - 1 Jan 2024
Zacharias Zeb R, Houtman Jon C D
Abstract excerpt
We have developed a 31-color panel to define the steady-state phenotype of T cells in human peripheral blood (Table 1). The panel presented here was optimized using cryopreserved peripheral blood mononuclear cells (PBMC). The markers included in this panel were chosen in order to characterize the steady-state phenotype of T cells and includes markers (CD45RA, CD45RO, CCR7, CD95) to distinguish the main subsets...
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