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Article

Synthetically useful variants of industrial lipases from Burkholderia cepacia and Pseudomonas fluorescens.

Organic & biomolecular chemistry - 25 Oct 2017

Yoshida Kazunori, Ono Masakazu, Yamamoto Takahiro, Utsumi Takashi, Koikeda Satoshi, Ema Tadashi

Abstract excerpt

Industrial enzymes lipase PS (LPS) and lipase AK (LAK), which originate from Burkholderia cepacia and Pseudomonas fluorescens, respectively, are synthetically useful biocatalysts. To strengthen their catalytic performances, we introduced two mutations into hot spots of the active sites (residues 287 and 290). The LPS_L287F/I290A double mutant showed high catalytic activity and enantioselectivity for poor...

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Identifiers

PMID: 28956057
DOI: 10.1039/c7ob01823a

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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