Isolation and Structural Studies of Mitochondria from Pea Roots.

Abstract excerpt

For structural and respiratory studies, isolation of intact and active mitochondria is essential. Here, we describe an isolation method which gave good yield and intact mitochondria from 2-week-old pea (Pisum sativum) roots grown hydroponically under standard growth conditions. We used Percoll gradient centrifugation for this isolation procedure. The yield of purified mitochondria was 50 μg/g FW. Isolated...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Cell Fractionation
• Share to Germination
• Share to Microscopy, Confocal