Highly efficient DNA-free gene disruption in the agricultural pest Ceratitis capitata by CRISPR-Cas9 ribonucleoprotein complexes.

doi:10.1038/s41598-017-10347-5

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Highly efficient DNA-free gene disruption in the agricultural pest Ceratitis capitata by CRISPR-Cas9 ribonucleoprotein complexes.

Scientific reports - 30 Aug 2017

Meccariello Angela, Monti Simona Maria, Romanelli Alessandra, Colonna Rita, Primo Pasquale, Inghilterra Maria Grazia, Del Corsano Giuseppe, Ramaglia Antonio, Iazzetti Giovanni, Chiarore Antonia, Patti Francesco, Heinze Svenia D, Salvemini Marco, Lindsay Helen, Chiavacci Elena, Burger Alexa, Robinson Mark D, Mosimann Christian, Bopp Daniel, Saccone Giuseppe

Abstract excerpt

The Mediterranean fruitfly Ceratitis capitata (medfly) is an invasive agricultural pest of high economic impact and has become an emerging model for developing new genetic control strategies as an alternative to insecticides. Here, we report the successful adaptation of CRISPR-Cas9-based gene disruption in the medfly by injecting in vitro pre-assembled, solubilized Cas9 ribonucleoprotein complexes (RNPs) loaded...

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PMID: 28855635
DOI: 10.1038/s41598-017-10347-5
PMCID: PMC5577161

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