Article
Isolation of Live Fibroblasts by Fluorescence-Activated Cell Sorting.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Leavitt Tripp, Hu Michael S, Longaker Michael T
Abstract excerpt
Flow cytometry is a powerful tool in cell biology in that it allows real-time characterization of cellular phenotypes. Additionally, through the process of fluorescence-activated cell sorting (FACS), living cells can be isolated for future in vitro experiments, including single cell analysis. Here we describe the isolation of live fibroblasts from the dermis of the skin in mice using FACS. This method circumvents...
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