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Article

Isolation of membrane vesicles from prokaryotes: a technical and biological comparison reveals heterogeneity

7 Jun 2017

Abstract excerpt

ABSTRACT Prokaryotes release membrane vesicles (MVs) with direct roles in disease pathogenesis. MVs are heterogeneous when isolated from bacterial cultures so Density Gradient Centrifugation (DGC) is valuable for separation of MV subgroups from contaminating material. Here we report the technical variability and natural biological heterogeneity seen between DGC preparations of MVs for Mycobacterium smegmatis and...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 28717421
DOI: 10.1080/20013078.2017.1324731

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