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A new isolation method for bacterial extracellular vesicles providing greater purity and improved proteomic detection of vesicle proteins

25 Apr 2023

Abstract excerpt

Abstract Contaminants within cell culture media often co‐isolate with eukaryotic extracellular vesicles (EVs) thus affecting their biological properties. It has yet to be investigated if this is also true for bacterial EVs (BEVs), especially for organisms grown in complex culture media containing animal‐derived products. To address this question, we isolated BEVs from the fastidious bacterium Helicobacter pylori...

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PMID: 38938280
DOI: 10.1002/jex2.84

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