Article
Selectable marker independent transformation of recalcitrant maize inbred B73 and sorghum P898012 mediated by morphogenic regulators BABY BOOM and WUSCHEL2.
Plant cell reports - 1 Sept 2017
Mookkan Muruganantham, Nelson-Vasilchik Kimberly, Hague Joel, Zhang Zhanyuan J, Kausch Albert P
Abstract excerpt
KEY MESSAGE: Discriminatory co-expression of maize BBM and WUS transcriptional factor genes promoted somatic embryogenesis and efficient Agrobacterium -mediated transformation of recalcitrant maize inbred B73 and sorghum P898012 genotypes without use of a selectable marker gene. The use of morphogenic regulators to overcome barriers in plant transformation is a revolutionary breakthrough for basic plant science...
Topics
- Agrobacterium tumefaciens
- Droughts
- Gene Expression Regulation, Plant
- Genetic Markers
- Genotype
- Green Fluorescent Proteins
- Models, Genetic
- Plant Proteins
- Plants, Genetically Modified
- Promoter Regions, Genetic
- Sorghum
- Transcription Factors