Article
Agrobacterium-mediated transformation of the temperate grass Brachypodium distachyon (genotype Bd21) for T-DNA insertional mutagenesis.
Plant biotechnology journal - 1 Apr 2008
Vain Philippe, Worland Barbara, Thole Vera, McKenzie Neil, Alves Silvia C, Opanowicz Magdalena, Fish Lesley J, Bevan Michael W, Snape John W
Abstract excerpt
Brachypodium distachyon is a promising model system for the structural and functional genomics of temperate grasses because of its physical, genetic and genome attributes. The sequencing of the inbred line Bd21 (http://www.brachypodium.org) started in 2007. However, a transformation method remains to be developed for the community standard line Bd21. In this article, a facile, efficient and rapid transformation...
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