Article
Screening potential SSR markers of the anadromous fish Coilia nasus by de novo transcriptome analysis using Illumina sequencing.
Genetics and molecular research : GMR - 13 Nov 2015
Fang D-A, Zhou Y-F, Duan J-R, Zhang M-Y, Xu D-P, Liu K, Xu P, Wei Q
Abstract excerpt
RNA-Seq technology has been widely applied to transcriptomics, genomics, molecular marker development, and functional gene studies. In the genome, microsatellites are simple sequence repeats (SSR) with a high degree of polymorphism that are used as DNA markers in many molecular genetic studies. Using traditional methods such as magnetic bead enrichment, only a few microsatellite markers have been isolated. Coilia...
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