Article
The influence of substrate on the spectral properties of oxyferrous wild-type and T252A cytochrome P450-CAM.
Archives of biochemistry and biophysics - 1 Apr 2005
Sono Masanori, Perera Roshan, Jin Shengxi, Makris Thomas M, Sligar Stephen G, Bryson Thomas A, Dawson John H
Abstract excerpt
To probe whether the nature of the substrate can directly influence the spectral properties of oxyferrous cytochrome P450-CAM, the complex has been investigated in the absence and in the presence of the natural substrate (1R)-camphor (camphor) and of several camphor analogs. The oxyferrous complex of T252A P450-CAM, a mutant lacking the hydroxyl group that forms a hydrogen bond to the heme iron-coordinated...
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