Article
The Generation of Doubled Haploid Lines for QTL Mapping.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Filiault Daniele L, Seymour Danelle K, Maruthachalam Ravi, Maloof Julin N
Abstract excerpt
Recombinant inbred lines (RILs) are an essential tool for quantitative trait locus (QTL) mapping in Arabidopsis thaliana. Conventionally, the development of these lines is a time-consuming and tedious process requiring six to eight generations of selfing. Here, we describe an alternative approach: the rapid generation of RILs in A. thaliana via the creation of doubled haploids. In this method, F1 plants are...
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