Article
A haploid genetics toolbox for Arabidopsis thaliana.
Nature communications - 31 Oct 2014
Ravi Maruthachalam, Marimuthu Mohan Prem Anand, Tan Ek Han, Maheshwari Shamoni, Henry Isabelle M, Marin-Rodriguez Brenda, Urtecho Guillaume, Tan Jie, Thornhill Kristina, Zhu Fan, Panoli Aneesh, Sundaresan Venkatesan, Britt Anne B, Comai Luca, Chan Simon W L
Abstract excerpt
Genetic analysis in haploids provides unconventional yet powerful advantages not available in diploid organisms. In Arabidopsis thaliana, haploids can be generated through seeds by crossing a wild-type strain to a transgenic strain with altered centromeres. Here we report the development of an improved haploid inducer (HI) strain, SeedGFP-HI, that aids selection of haploid seeds prior to germination. We also show...
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