Culture independent analysis using gnd as a target gene to assess Escherichia coli diversity and community structure.

Abstract excerpt

Current culture methods to investigate changes in Escherichia coli community structure are often slow and laborious. Genes such as gnd (6-phosphogluconate dehydrogenase) have a highly variable nucleotide sequence and may provide a target for E. coli microbiome analysis using culture-independent methods. Metabarcoded PCR primers were used to generate separate libraries from calf faecal samples for high throughput...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Cattle
• Share to Escherichia coli