Article
Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion.
Scientific reports - 28 Mar 2017
Nekrasov Vladimir, Wang Congmao, Win Joe, Lanz Christa, Weigel Detlef, Kamoun Sophien
Abstract excerpt
Genome editing has emerged as a technology with a potential to revolutionize plant breeding. In this study, we report on generating, in less than ten months, Tomelo, a non-transgenic tomato variety resistant to the powdery mildew fungal pathogen using the CRISPR/Cas9 technology. We used whole-genome sequencing to show that Tomelo does not carry any foreign DNA sequences but only carries a deletion that is...
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