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Article

Gene delivery to Nile tilapia cells for transgenesis and the role of PI3K-c2α in angiogenesis.

Scientific reports - 20 Mar 2017

Tonelli Fernanda Maria Policarpo, Lacerda Samyra Maria Dos Santos Nassif, Procópio Marcela Santos, Lemos Breno Luiz Sales, de França Luiz Renato, Resende Rodrigo Ribeiro

Abstract excerpt

Microinjection is commonly performed to achieve fish transgenesis; however, due to difficulties associated with this technique, new strategies are being developed. Here we evaluate the potential of lentiviral particles to genetically modify Nile tilapia cells to achieve transgenesis using three different approaches: spermatogonial stem cell (SSC) genetic modification and transplantation (SC), in vivo transduction...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 28317860
DOI: 10.1038/srep44317
PMCID: PMC5357942

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