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Assessment of human sperm DNA integrity using two cytochemical tests: Acridine orange test and toluidine blue assay

22 Feb 2017

Abstract excerpt

Primary infertility affects approximately 15% of couples, with male factor infertility accounting for 50% of cases. Semen samples from 41 patients with asthenoteratospermia and 28 men with proven fertility were analysed according to World Health Organization guidelines. Abnormal sperm chromatin structure was assessed by toluidine blue assay (TBA), and DNA denaturation (DD) was detected by the acridine orange test...

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PMID: 28224638
DOI: 10.1111/and.12765

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