Article
Assays to Investigate the N-Glycosylation State and Function of Plant Pattern Recognition Receptors.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017
Lawrence Stacey A, Ceserani Teresa, Clay Nicole K
Abstract excerpt
The biogenesis and functionality of pattern recognition receptors (PRRs) are critical for robust plant immune responses. Here, we present methods to determine the N-glycosylation state and ligand-induced activity of these receptors for comparative quantitative analysis. These techniques can be used to identify mutants and chemical inhibitors affecting PRR biogenesis and functionality. When combined, these...
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