Article
Knockdown of a cellulose synthase gene BoiCesA affects the leaf anatomy, cellulose content and salt tolerance in broccoli.
Scientific reports - 7 Feb 2017
Li Shuangtao, Zhang Lei, Wang Ying, Xu Fengfeng, Liu Mengyun, Lin Peng, Ren Shuxin, Ma Rui, Guo Yang-Dong
Abstract excerpt
Cellulose is the major component of cell wall materials. A 300 bp specific fragment from the cDNA fragment was chosen to insert into vector pFGC1008 at forward and reverse orientations to construct the recombinant RNAi vector. Knockdown of BoiCesA caused "dwarf" phenotype with smaller leaves and a loss of the content of cellulose. Moreover, RT-PCR analysis confirmed that the expression of the RNAi apparatus could...
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