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Article

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation.

Journal of visualized experiments : JoVE - 4 Jan 2017

Erdogan Fettah, Lento Cristina, Yaseen Ayat, Nowroozi-Dayeni Roksana, Kheyson Sasha, Audette Gerald F

Abstract excerpt

The transfer of genetic material by bacterial conjugation is a process that takes place via complexes formed by specific transfer proteins. In Escherichia coli, these transfer proteins make up a DNA transfer machinery known as the mating pair formation, or DNA transfer complex, which facilitates conjugative plasmid transfer. The objective of this paper is to provide a method that can be used to determine the role...

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Source

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Identifiers

PMID: 28117821
DOI: 10.3791/54854
PMCID: PMC5351875

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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