Article
Use of Nicotiana tabacum transplastomic plants engineered to express a His-tagged CP47 for the isolation of functional photosystem II core complexes.
Plant physiology and biochemistry : PPB - 1 Feb 2017
Pagliano Cristina, Bersanini Luca, Cella Rino, Longoni Paolo, Pantaleoni Laura, Dass Abhishek, Leelavathi Sadhu, Reddy Vanga Siva
Abstract excerpt
This work focuses on the development of a molecular tool for purification of Photosystem II (PSII) from Nicotiana tabacum (L.). To this end, the chloroplast psbB gene encoding the CP47 PSII subunit was replaced with an engineered version of the same gene containing a C-terminal His-tag. Molecular analyses assessed the effective integration of the recombinant gene and its expression. Despite not exhibiting any...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
