Article
Isolation of highly active photosystem II core complexes with a His-tagged Cyt b559 subunit from transplastomic tobacco plants.
Biochimica et biophysica acta - 1 Dec 2008
Fey Holger, Piano Dario, Horn Ruth, Fischer David, Schmidt Matthias, Ruf Stephanie, Schröder Wolfgang P, Bock Ralph, Büchel Claudia
Abstract excerpt
Photosystem II (PSII) is a huge multi-protein-complex consisting, in higher plants and green algae, of the PS II core and the adjacent light harvesting proteins. In the study reported here, N-terminal His-tags were added to the plastome-encoded alpha-subunit of cytochrome b559, PsbE, in tobacco plants, thus facilitating rapid, mild purification of higher plant PSII. Biolistic chloroplast transformation was used...
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