Article
Development of Commercial Thermo-sensitive Genic Male Sterile Rice Accelerates Hybrid Rice Breeding Using the CRISPR/Cas9-mediated TMS5 Editing System.
Scientific reports - 22 Nov 2016
Zhou Hai, He Ming, Li Jing, Chen Liang, Huang Zhifeng, Zheng Shaoyan, Zhu Liya, Ni Erdong, Jiang Dagang, Zhao Bingran, Zhuang Chuxiong
Abstract excerpt
Hybrid rice breeding offers an important strategy to improve rice production, in which the cultivation of a male sterile line is the key to the success of cross-breeding. CRISPR/Cas9 systems have been widely used in target-site genome editing, whereas their application for crop genetic improvement has been rarely reported. Here, using the CRISPR/Cas9 system, we induced specific mutations in TMS5, which is the...
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