Article
Subtyping Escherichia coli Virulence Genes Isolated from Feces of Beef Cattle and Clinical Cases in Alberta.
Foodborne pathogens and disease - 1 Jan 2017
Tostes Renata, Goji Noriko, Amoako Kingsley, Chui Linda, Kastelic John, DeVinney Rebekah, Stanford Kim, Reuter Tim
Abstract excerpt
Clinical outcomes of Shiga toxin (stx)-producing Escherichia coli infection are largely determined by virulence gene subtypes. This study used a polymerase chain reaction (PCR)-pyrosequencing assay to analyze single-nucleotide polymorphisms for subtyping three major virulence genes (stx1, stx2, eae) of pathogenic E. coli (O157, O26, O111, and O103) isolated from cattle over a 2-year interval (n = 465) and human...
Topics
- Alberta
- Animals
- Carbohydrate Epimerases
- Cattle
- Escherichia coli O157
- Feces
- Food Contamination
- Food Microbiology
- Genes, Bacterial
- Humans
- Polymorphism, Single Nucleotide