Article
Generating Rho-0 Cells Using Mesenchymal Stem Cell Lines.
PloS one - 1 Jan 2016
Fernández-Moreno Mercedes, Hermida-Gómez Tamara, Gallardo M Esther, Dalmao-Fernández Andrea, Rego-Pérez Ignacio, Garesse Rafael, Blanco Francisco J
Abstract excerpt
INTRODUCTION: The generation of Rho-0 cells requires the use of an immortalization process, or tumor cell selection, followed by culture in the presence of ethidium bromide (EtBr), incurring the drawbacks its use entails. The purpose of this work was to generate Rho-0 cells using human mesenchymal stem cells (hMSCs) with reagents having the ability to remove mitochondrial DNA (mtDNA) more safely than by using...
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