Article
A new Bradyrhizobium japonicum gene required for free-living growth and bacteroid development is conserved in other bacteria and in plants.
Molecular plant-microbe interactions : MPMI - 1 Jan 2000
Weidenhaupt M, Schmid-Appert M, Thöny B, Hennecke H, Fischer H M
Abstract excerpt
In the nitrogen-fixing soybean symbiont Bradyrhizobium japonicum, a new DNA region, orf74, was discovered which is required for optimal free-living growth and, by consequence, also necessary for the formation of an effective symbiosis. A Tn5-233 insertion of orf14 resulted in a mutant, strain 74, that has a reduced growth rate in free-living cultures under all conditions tested and less than 1% residual symbiotic...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Conserved Sequence
- DNA Transposable Elements
- DNA, Bacterial
- Escherichia coli
- Genetic Complementation Test
- Microscopy, Electron
- Molecular Sequence Data