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Article

Rapid identification and expression of human TCRs in retrogenic mice.

Journal of immunological methods - 1 Dec 2016

Sprouse Maran L, Blahnik Gabriele, Lee Thomas, Tully Natalie, Banerjee Pinaki, James Eddie A, Redondo Maria J, Bettini Matthew L, Bettini Maria

Abstract excerpt

Single-cell paired TCR identification is a powerful tool, but has been limited in its previous incompatibility with further functional analysis. The current protocol describes a method to clone and functionally evaluate in vivo TCRs derived from single antigen-responsive human T cells and monoclonal T cell lines. We have improved upon current PCR-based TCR sequencing protocols by developing primers that allow...

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Source

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Identifiers

PMID: 27589924
DOI: 10.1016/j.jim.2016.08.010
PMCID: PMC5439214

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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