Article
Concomitant Evaluation of a Panel of Exosome Proteins and MiRs for Qualification of Cultured Human Corneal Endothelial Cells.
Investigative ophthalmology & visual science - 1 Aug 2016
Ueno Morio, Asada Kazuko, Toda Munetoyo, Nagata Kazue, Sotozono Chie, Kosaka Nobuyoshi, Ochiya Takahiro, Kinoshita Shigeru, Hamuro Junji
Abstract excerpt
PURPOSE: We elucidate a method to use secreted miRNA profiles to qualify cultured human corneal endothelial cells (cHCECs) adaptable for cell-injection therapy. METHODS: The variations of cHCECs in their composites of heterogeneous subpopulations (SPs) were verified in relation to their surface cluster-of-differentiation (CD) markers. Integrated analysis of micro RNA (miRNA) profiles in culture supernatants (CS)...
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