Article
Comparative Evaluation of Real-Time PCR Methods for Human Noroviruses in Wastewater and Human Stool.
PloS one - 1 Jan 2016
Masago Yoshifumi, Konta Yoshimitsu, Kazama Shinobu, Inaba Manami, Imagawa Toshifumi, Tohma Kentaro, Saito Mayuko, Suzuki Akira, Oshitani Hitoshi, Omura Tatsuo
Abstract excerpt
Selecting the best quantitative PCR assay is essential to detect human norovirus genome effectively from clinical and environmental samples because no cell lines have been developed to propagate this virus. The real-time PCR methods for noroviruses GI (4 assays) and GII (3 assays) were evaluated using wastewater (n = 70) and norovirus-positive stool (n = 77) samples collected in Japan between 2012 and 2013....
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