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Differentiation of entC1 from entC2/entC3 with a single primer pair using simple and rapid SYBR Green-based RT-PCR melt curve analysis.

Applied microbiology and biotechnology - 1 Oct 2016

Nagaraj Sowmya, Ramlal Shylaja, Venkataswamachari Bhavani Peddayelachagiri, Paul Soumya, Kingston Joseph, Batra Harsh Vardhan

Abstract excerpt

In spite of their involvement in foodborne illness, the epidemiological relevance of staphylococcal enterotoxin C (SEC) subtypes is poorly documented may be due to high sequence similarity. Among subtypes, SEC1, SEC2, and SEC3 exhibit more than 97 % homology because of which specific detection tools are seldom available to identify and differentiate them. In this study, a SYBR Green-based RT-PCR followed by melt...

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PMID: 27245675
DOI: 10.1007/s00253-016-7637-y

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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