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One-step duplex RT-droplet digital PCR assay for the detection of norovirus GI and GII in lettuce and strawberry.

Food microbiology - 1 Apr 2021

Sun Chongzhen, Chen Jiayin, Li Hui, Fang Ling, Wu Shiwei, Jayavanth Pallavi, Tang Shuze, Sanchez Gloria, Wu Xiyang

Abstract excerpt

The study was designed to develop a sensitive one-step duplex reverse transcription droplet digital polymerase chain reaction (RT-ddPCR) to detect norovirus genogroup I and II (NoV GI and GII) in lettuce and strawberry. The specificity, sensitivity, repeatability and robustness of the assay was compared with RT-qPCR. The lowest concentration detected by RT-ddPCR for NoV GI and NoV GII were 4.68 and 8.47 copies/μL...

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Identifiers

PMID: 33279078
DOI: 10.1016/j.fm.2020.103653

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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