Article
De novo construction of a "Gene-space" for diploid plant genome rich in repetitive sequences by an iterative Process of Extraction and Assembly of NGS reads (iPEA protocol) with limited computing resources.
BMC research notes - 11 Feb 2016
Aluome Christelle, Aubert Grégoire, Alves Carvalho Susete, Le Paslier Marie-Christine, Burstin Judith, Brunel Dominique
Abstract excerpt
BACKGROUND: The continuing increase in size and quality of the "short reads" raw data is a significant help for the quality of the assembly obtained through various bioinformatics tools. However, building a reference genome sequence for most plant species remains a significant challenge due to the large number of repeated sequences which are problematic for a whole-genome quality de novo assembly. Furthermore,...
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