Article
Identification of a novel DFA I-producing inulin fructotransferase from Streptomyces davawensis.
International journal of biological macromolecules - 1 Nov 2016
Zhu Yingying, Yu Shuhuai, Huang Danyang, Zhang Tao, Jiang Bo, Mu Wanmeng
Abstract excerpt
In this work, a novel gene encoding DFA I-forming inulin fructotransferase (IFTase) from Streptomyces davawensis SK39.001 was cloned and expressed in Escherichia coli. The enzyme was purified, identified, and characterized. The results showed that this IFTase (DFA I-forming) is a trimer (molecular weight of 125KDa) consisting of three identical subunits (the molecular weight as assayed by SDS-PAGE was...
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