Article
Evaluation of epithelial-mesenchymal transitioned circulating tumor cells in patients with resectable gastric cancer: Relevance to therapy response.
World journal of gastroenterology - 21 Dec 2015
Li Ting-Ting, Liu Hao, Li Feng-Ping, Hu Yan-Feng, Mou Ting-Yu, Lin Tian, Yu Jiang, Zheng Lei, Li Guo-Xin
Abstract excerpt
AIM: To evaluate the epithelial-to-mesenchymal transition (EMT) of circulating tumor cells (CTCs) in gastric cancer patients. METHODS: We detected tumor cells for expression of four epithelial (E(+)) transcripts (keratins 8, 18, and 19 and epithelial cell adhesion molecule) and two mesenchymal (M(+)) transcripts (Vimentin and Twist) by a quantifiable, dual-colorimetric RNA-in situ hybridization assay. Between...
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