Article
Generation of artificial drooping leaf mutants by CRISPR-Cas9 technology in rice.
Genes & genetic systems - 1 Jan 2016
Ikeda Takuyuki, Tanaka Wakana, Mikami Masafumi, Endo Masaki, Hirano Hiro-Yuki
Abstract excerpt
CRISPR-Cas9 technology, which uses an RNA-guided nuclease, has been developed as an efficient and versatile genome-editing method to induce mutations in genes of interest. To examine the feasibility of this method in developmental studies of a model monocot, rice (Oryza sativa), we introduced the construct gDL-1, which produced a guide RNA targeting the DROOPING LEAF (DL) gene. DL regulates midrib formation in...
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