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Fission Yeast Cell Cycle Synchronization Methods.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2016

Tormos-Pérez Marta, Pérez-Hidalgo Livia, Moreno Sergio

Abstract excerpt

Fission yeast cells can be synchronized by cell cycle arrest and release or by size selection. Cell cycle arrest synchronization is based on the block and release of temperature-sensitive cell cycle mutants or treatment with drugs. The most widely used approaches are cdc10-129 for G1; hydroxyurea (HU) for early S-phase; cdc25-22 for G2, and nda3-KM311 for mitosis. Cells can also be synchronized by size selection...

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PMID: 26519320
DOI: 10.1007/978-1-4939-3145-3_20

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