Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A novel immunofluorescent assay to investigate oxidative phosphorylation deficiency in mitochondrial myopathy: understanding mechanisms and improving diagnosis.

Scientific reports - 15 Oct 2015

Rocha Mariana C, Grady John P, Grünewald Anne, Vincent Amy, Dobson Philip F, Taylor Robert W, Turnbull Doug M, Rygiel Karolina A

Abstract excerpt

Oxidative phosphorylation defects in human tissues are often challenging to quantify due to a mosaic pattern of deficiency. Biochemical assays are difficult to interpret due to the varying enzyme deficiency levels found in individual cells. Histochemical analysis allows semi-quantitative assessment of complex II and complex IV activities, but there is no validated histochemical assay to assess complex I activity...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Respiration
Share to Electron Transport

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Electron Transport Chain Complex Proteins

Share to Fluorescent Antibody Technique

Share to Mitochondrial Myopathies

Share to Observer Variation

Share to Oxidative Phosphorylation

Share to Succinate Dehydrogenase

Source

Open in PubMed Open full text

Identifiers

PMID: 26469001
DOI: 10.1038/srep15037
PMCID: PMC4606788

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A novel immunofluorescent assay to investigate oxidative phosphorylation deficiency in mitochondrial myopathy: understanding mechanisms and improving diagnosis.PMID 26469001Read ↗

Using a quantitative quadruple immunofluorescent assay to diagnose isolated mitochondrial Complex I deficiency.Semantic proximity Read ↗

Decoding mitochondrial heterogeneity in single muscle fibres by imaging mass cytometrySemantic proximity Read ↗

Immunohistochemical analysis of the oxidative phosphorylation complexes in skeletal muscle from patients with mitochondrial DNA encoded tRNA gene defects.Semantic proximity Read ↗

A novel histochemistry assay to assess and quantify focal cytochrome c oxidase deficiency.Semantic proximity Read ↗

Isolated deficiencies of OXPHOS complexes I and IV are identified accurately and quickly by simple enzyme activity immunocapture assays.Semantic proximity Read ↗

Integrated single cell functional-proteomic profiling of human skeletal muscle reveals a shift in cellular specificity in nemaline myopathySemantic proximity Read ↗

Mitochondrial DysfunctionSemantic proximity Read ↗

Exercise Testing, Physical Training and Fatigue in Patients with Mitochondrial Myopathy Related to mtDNA MutationsSemantic proximity Read ↗

Single fiber proteomics of respiratory chain defects in mitochondrial disordersSemantic proximity Read ↗

The clinical utility of functional testing in fibroblasts to diagnose primary mitochondrial diseaseSemantic proximity Read ↗

Mitochondrial oxidative phosphorylation: pitfalls and tips in measuring and interpreting enzyme activities.Semantic proximity Read ↗

Mitochondrial dysfunction in myofibrillar myopathy.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.