Article
Mechanical Stabilization of Mouse Carotid Artery for In Vivo Intravital Microscopy Imaging of Atherogenesis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Chèvre Raphaël
Abstract excerpt
We present here a procedure that allows real-time high-resolution multichannel imaging of early atherosclerotic lesions of live mice, by dramatically reducing the respiratory and pulsatile movements of the athero-susceptible carotid artery, without significantly altering blood flow dynamics. This surgical preparation can be combined with the use of various fluorescent probes and reporter mice to simultaneously...
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