Article
Outbred genome sequencing and CRISPR/Cas9 gene editing in butterflies.
Nature communications - 10 Sept 2015
Li Xueyan, Fan Dingding, Zhang Wei, Liu Guichun, Zhang Lu, Zhao Li, Fang Xiaodong, Chen Lei, Dong Yang, Chen Yuan, Ding Yun, Zhao Ruoping, Feng Mingji, Zhu Yabing, Feng Yue, Jiang Xuanting, Zhu Deying, Xiang Hui, Feng Xikan, Li Shuaicheng, Wang Jun, Zhang Guojie, Kronforst Marcus R, Wang Wen
Abstract excerpt
Butterflies are exceptionally diverse but their potential as an experimental system has been limited by the difficulty of deciphering heterozygous genomes and a lack of genetic manipulation technology. Here we use a hybrid assembly approach to construct high-quality reference genomes for Papilio xuthus (contig and scaffold N50: 492 kb, 3.4 Mb) and Papilio machaon (contig and scaffold N50: 81 kb, 1.15 Mb), highly...
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